客户科研成果列表


基因合成 | 基因克隆 | sgrna文库 | 密码子优化| 引物合成| 探针合成| 测序| 载体构建| dna存储| rna干扰

基因合成

  1. peng c., et al. selective carboxylation of substituted phenols with engineered escherichia coli whole-cells. tetrahedron letters, september, 2018. doi: 10.1016/j.tetlet.2018.09.019.
  2. jian s., et al. determining allele-specific protein expression (aspe) using a novel qconcat-based proteomics method. journal of proteome research, august 24th, 2018. doi: 10.1021/acs.jproteome.8b00620.
  3. jinghua w., et al. car-t cells targeting cll-1 as an approach to treat acute myeloid leukemia. journal of hematology & oncology, 2018. 11:7. doi: 10.1186/s13045-017-0553-5.
  4. gong s., wu c. generation of fabs-in-tandem immunoglobulin molecules for dual-specific targeting. methods, aug 3, 2018. pii: s1046-2023(18)30105-1. doi: 10.1016/j.ymeth.2018.07.014.
  5. xiu, h., et al., microrna-485-5p functions as a tumor suppressor in colorectal cancer cells by targeting cd147. journal of cancer. 2018; 9(15):2603-2611. doi:10.7150/jca.24918.
  6. yang, x., et al., expression of phosphotransacetylase in rhodosporidium toruloides leading to improved cell growth and lipid production. royal society of chemistry, 10th july 2018. 8:24673-24678. doi: 10.1039/c8ra03028f.
  7. shi, j., et al., a novel qconcat-based proteomics method for determining allele-specific protein expression (aspe): a new approach to identify cis-acting genetic variants. biorxiv, 2018. doi: 10.1101/264614.
  8. su, j., et al., galectin-10: a new structural type of prototype galectin dimer and effects on saccharide ligand binding. glycobiology, 2018. 28(3): p. 159-168. doi:0.1093/glycob/cwx107
  9. wang, x., et al., visualizing soluble protein mutants by using monomeric red fluorescent protein as a reporter for directed evolution. applied biochemistry and biotechnology, 2018. 185(1): p.81-90. doi: 10.1007/ s12010-017-2640-z.
  10. lu, h., et al., identification of a l-lactate dehydrogenase with 3, 4-dihydroxyphenylpyruvic reduction activity for l-danshensu production. process biochemistry, 2018. doi: 10.1016/j.procbio.2018.06.011
  11. jin, w., et al., increased soluble heterologous expression of a rat brain 3-o-sulfotransferase 1 – a key enzyme for heparin biosesynthesis. protein expression and purification, 2018, vol.151, p.23-29. doi: 10.1016/j.pep.2018.06.007
  12. li j, zhang y, zhang y, et al. gsnor modulates hyperhomocysteinemia-induced t cell activation and atherosclerosis by switching akt s-nitrosylation to phosphorylation[j]. redox biology, 2018. doi.org/10.1016/j.redox.2018.04.021
  13. cheng y, chen g, wang l, et al. triptolide-induced mitochondrial damage dysregulates fatty acid metabolism in mouse sertoli cell s[j]. toxicology letters, 2018, 292: 136-150. doi: 10.1016/j.toxlet.2018.04.035.
  14. pan y, sun h, hu x, et al. the inhibitory role of mir 485 5p in colorectal cancer proliferation and invasion via targeting of cd147 [j]. oncology reports, 2018, 39(5): 2201-2208. doi: 10.3892/or.2018.6309.
  15. zhang w, he z, yi l, et al. a dual-functional microfluidic chip for on-line detection of interleukin-8 based on rolling circle amplification[j]. biosesensors and bioelectronics, 2018, 102: 652-660. doi: 10.1016/j.bioses.2017.12.017.
  16. chang z, meng f, zhang z, et al. microrna‐193b‐3p regulates matrix metalloproteinase 19 expression in interleukin‐1β‐induced human chondrocytes[j]. journal of cellular biochemistry, 2018, 119(6): 4775-4782. doi: 10.1002/jcb.26669.
  17. he, p., et al., development and application of a duplex real-time rt-pcr assay for simultaneous detection of coxsackievirus a6 and coxsackievirus a10. annals of clinical and laboratory science, 2017. 47(2): p. 178-183. pmid: 28442520
  18. wang, li., et al., increased processivity, misincorporation, and nucleotide incorporation efficiency in sulfolobus solfataricus dpo4 thumb domain mutants. applied and environmental microbiology, 2017. 83(18): p. e01013-17. doi: 10.1128/aem.01013-17.
  19. wang, x., et al., engineering escherichia coli nicotinic acid mononucleotide adenylyltransferase for fully active amidated nad biosesynthesis. applied and environmental microbiology, 2017. 83(13): p. e00692-17. doi: 10.1128/aem.00692-17.
  20. xie, z., et al., design and chemical synthesis of eukaryotic chromosomes. chemical society reviews, 2017. 46(23): p. 7191-7207. doi:10.1039/ c7cs00208ds
  21. dai, s., et al., hnrnpa2b1 regulates the epithelial-mesenchymal transition in pancreatic cancer cells through the erk/snail signalling pathway. cancer cell int, 2017. 17: p. 12. doi: 10.1186/s12935-016-0368-4.
  22. jin, l., et al., icaritin induces mitochondrial apoptosis by up-regulating mir-124 in human oral squamous cell carcinoma cells. biomed pharmacother, 2017. 85: p. 287-295. doi: 10.1016/j.biopha.2016.11.023.
  23. li, n., et al., a dna-directed covalent conjugation fluorescence probe for in vitro detection of functional matrix metalloproteinases. analyst, 2017. doi: 10.1039/c6an02339h
  24. liu j, chen w, zhang h, et al. mir 214 targets the pten mediated pi3k/akt signaling pathway and regulates cell proliferation and apoptosis in ovarian cancer[j]. oncology letters, 2017, 14(5): 5711-5718. doi: 10.3892/ol.2017.6953
  25. duan l, ding w, liu x, et al. biosesynthesis and engineering of kaempferol in saccharomyces cerevisiae[j]. microbial cell factories, 2017, 16(1): 165. doi: 10.1186/s12934-017-0774-x
  26. zhao z, xue j, song y, et al. downregulated long non coding rna tcons_00068220 upregulates apoptosis in gastric cancer cells[j]. oncology letters, 2017, 14(5): 6143-6150. doi: 10.3892/ol.2017.6977
  27. si, x., et al., upregulation of mir-99a is associated with poor prognosis of acute myeloid leukemia and promotes myeloid leukemia cell expansion. oncotarget, 2016. 7(47): p. 78095-78109. doi: 10.18632/ oncotarget.12947.
  28. na, r., et al., indentification of vincamine indole alkaloids producing endophytic fungi isolated from nerium indicum, apocynaceae. microbiol res, 2016. 192: p. 114-21. doi: 10.1016/j.micres.2016.06.008.
  29. pan, x., et al., physcion induces apoptosis in hepatocellular carcinoma by modulating mir-370. am j cancer res, 2016. 6(12): p. 2919-2931. pmcid: pmc5199765
  30. li, z., et al., ns5atp6 modulates intracellular triglyceride content through fgf21 and independently of sirt1 and srebp1. biochem biophys res commun, 2016. 475(1): p. 133-9. doi: 10.1016/j.bbrc.2016.05.056.
  31. yang, h., et al., optimized expression of prolyl aminopeptidase in pichia pastoris and its characteristics after glycosylation. world j microbiol biotechnol, 2016. 32(11): p. 176. doi: 10.1007/s11274-016-2135-z.
  32. tang, y., et al., radiation-induced mir-208a increases the proliferation and radioresistance by targeting p21 in human lung cancer cells. j exp clin cancer res, 2016. 35: p. 7.doi: 10.1186/s13046-016-0285-3
  33. zhi n, wan z, liu x, et al. codon optimization of human parvovirus b19 capsid genes greatly increases their expression in nonpermissive cells[j]. journal of virology, 2010, 84(24): 13059-13062.doi: 10.1128/jvi.00912-10

基因克隆

  1. hu, j., et al., clone and functional analysis of seryl-trna synthetase and tyrosyl-trna synthetase from silkworm, bombyx mori. sci rep, 2017. 7: p. 41563. doi: 10.1038/srep41563.
  2. yan, h., et al., crif1 enhances p53 activity via the chromatin remodeler snf5 in the hct116 colon cancer cell lines. bba – gene regulatory mechanisms.doi: 10.1016/j.bbagrm

sgrna文库

  1. lu, y., et al., genome-wide targeted mutagenesis in rice using the crispr/cas9 system. mol plant, 2017.doi: 10.1016/j.molp.2017.06.007.

密码子优化

  1. liu, b., et al., codon optimization significantly enhanced the expression of human 37-kda ilrp in escherichia coli. biotech, 2018. 8(4): p. 210. doi:10.1007/s13205-018-1234-y.
  2. jin, w., et al., increased soluble heterologous expression of a rat brain 3-o-sulfotransferase 1 – a key enzyme for heparin biosesynthesis. protein expression and purification, 2018, vol.151, p.23-29. doi: 10.1016/j.pep.2018.06.007
  3. peng, z., et al., high and efficient isomaltulose production using an engineered yarrowia lipolytica strain. bioresource technology, june, 2018. doi: 10.1016/j.biortech.2018.06.081

引物合成

  1. haiying n., et al., role of mir-214 in modulating proliferation and invasion of human colon cancer sw620 cells. oncology letters, september 28th, 2018. doi: 10.3892/ol.2018.9521.
  2. jian w., et al., amphiregulin potentiates airway inflammation and mucus hypersecretion induced by urban particulate matter via the egfr-pi3kα-akt/erk pathway. cellular signalling, january, 2019. doi: 10.1016/j.cellsig.2018.10.002.
  3. fang, b., et al., luteolin inhibits angiogenesis of the m2‑like tams via the downregulation of hypoxia inducible factor‑1α and the stat3 signalling pathway under hypoxia. molecular medicine reports, july 3, 2018. doi: 10.3892/mmr.2018.9250.
  4. chen, s., et al. ubiquitin-conjugating enzyme ube2o regulates cellular clock function by promoting the degradation of the transcription factor bmal1. journal of biology chemistry, 2018. doi: 10.1074/jbc.r a117.001432.
  5. sun, y., et al., effect on the liver cancer cell invasion ability by studying the associations between autophagy and trap1 expression. oncology letters, 2018. doi: 10.3892/ol.2018.8774
  6. wang, j., et al., transcriptome profiling reveals phlda1 as a novel molecular marker for ischemic cardiomyopathy. journal of molecular neuroscience, 2018. 65(1): p. 102-109. doi: 10.1007/s12031-018-1066-6.
  7. zhang, h., et al., genetic polymorphism in the ryr1 c6487t is associated with severity of hypospadias in chinese han children. biomed research international, 2018. article id 7397839. doi: 10.1155/2018/7397839.
  8. wang, j., et al., urban particulate matter triggers lung inflammation via the ros-mapk-nf-κb signaling pathway. journal of thoracic disease, 2017. 9(11): p. 4398-4412. doi: 10.21037/jtd.2017.09.135.
  9. yu, d., et al., quercetin inhibits epithelial-mesenchymal transition, decreases invasiveness and metastasis, and reverses il-6 induced epithelial-mesenchymal transition, expression of mmp by inhibiting stat3 signaling in pancreatic cancer cells. oncotargets and therapy, 2017. 10: p. 4719-4729. doi: 10.2147/ott.s136840.
  10. zhang, z., et al., non-invasive detection of gastric cancer relevant d-amino acids with luminescent dna/silver nanoclusters. nanoscale, 2017. 9(48): p. 19367-19373. doi: 10.1039/c7nr07337b.
  11. zhang, y., et al., genetic and immune features of resectable malignant brainstem gliomas. oncotarget, 2017. 8(47): p. 82571-82582. doi: 10.18632/oncotarget.19653.
  12. wang, q., et al., physcion 8-o-β-glucopyranoside induces apoptosis, suppresses invasion and inhibits epithelial to mesenchymal transition of hepatocellular carcinoma hepg2 cells. biomedicine & pharmacotherapy, 2016. 83: p. 372-380. doi:10.1016/j.biopha.2016.06.045.

探针合成

  1. eastwood, m., et al., microrna 200b is upregulated in the lungs of fetal rabbits with surgically induced diaphragmatic hernia. prenatal diagnosis, 2018. doi: 10.1002/pd.5318.
  2. wu, y., et al. a cyclic catalysis enhanced electrochemiluminescence aptasensor based 3d graphene/photocatalysts cu2o-mwcnts. electochimica acta, 2018. 282: p. 672-679. doi:10.1016/ j.electacta.2018.06.104.
  3. zhang, y., et al., genetic and immune features of resectable malignant brainstem gliomas. oncotarget, 2017. 8(47): p. 82571-82582. doi: 10. 18632/oncotarget.19653.

测序

  1. chen, m., et al., molecular cloning and characterization of c1 tetrahydrofolate (c1-thf) synthase in bombyx mori, silkworm. gene, 2018. 663: p. 25-33. doi:10.1016/j.gene.2018.04.037
  2. yang, f., et al., local polyploidy phenomenon in escherichia coli and its significance in genetic engineering. biorxiv, 2018. doi: 10.1101/335448
  3. cheng, h., et al., the hyperthermophilic α-amylase from thermococcus sp. hj21 does not require exogenous calcium for thermostability because of high-binding affinity to calcium. journal of microbiology, 2017. 55(5): p. 379-387.doi: 10.1007/s12275-017-6416-5.

载体构建

  1. chu j., et al., mir-4319 suppress the malignancy of triple-negative breast cancer by regulating self-renewal and tumorigenesis of stem cells. cell physiol biochem. 18th jul 2018. 48(2): p.593-604. doi: 10.1159/000491888.
  2. bellido, f., et al., association between germline mutations in brf1, a subunit of the rna polymerase iii transcription complex, and hereditary colorectal cancer. gastroenterology, 2018. 154(1): p. 181-194. doi: 10.1053/j.gastro.2017.09.005.
  3. xu, x., et al., downregulation of hur inhibits the progression of esophageal cancer through interleukin-18. cancer res treat, 2018. 50(1): p.71-87. doi: 10.4143/crt.2017.013.

dna存储

  1. zhong, c., et al., a new digital information storing and reading system based on synthetic dna. science china. life sciences, 2018. 6: p. 1-3. doi: 10.1007/s11427-017-9131-7.

rna干扰

  1. ren, y., et al., role of c-abl- gsk3β signaling in mpp+-induced autophagy-lysosomal dysfunction. toxicological sciences, 2018. kfy155. doi:10.1093/toxsci/kfy155.

相关服务订购

  1. email:请将您的详细需求发送到support@synbio-tech.com
  2. 电话订购:您可以直接拨打免费热线4000-973-630转803联系经验丰富的工程师
  3. qq咨询:任何技术问题均可与在线互动,泓迅科技官方企业qq:4000-973-630